TABLE 2.
Oligonucleotides
Primer | Sequence | Use |
---|---|---|
ACT1 | 5′-CTATTGGTAACGAAAGATTCAG-3′ | qRT-PCR |
5′-CCTTACGGACATCGACATCA-3′ | ||
CTH2 | 5′-AGGTATGCTGCTGGAGCTGT-3′ | qRT-PCR |
5′-GAGGGCCATGAAGGTATCAA-3′ | ||
FET4 | 5′-GGAGAACTGCCTGTGGAAAA-3′ | qRT-PCR |
5′-TTCTCCGGTGTAAGGTGGAG-3′ | ||
FET3 | 5′-ACGGTGTGAATTACGCCTTC-3′ | qRT-PCR |
5′-TTGGAAAGCGTGACCATGTA-3′ | ||
ROX1 | 5′-AGGGCTTACAACCGGAAGAT-3′ | qRT-PCR |
5′-GCTGTTGCTCGATTTCCTTC-3′ | ||
CUP1 | 5′-TGAAGGTCATGAGTGCCAAT-3′ | qRT-PCR |
5′-GCATTTGTCGTCGCTGTTAC-3′ | ||
ARN2 | 5′-AGGTATGCTGCTGGAGCTGT-3′ | qRT-PCR |
5′-GAGGGCCATGAAGGTATCAA-3′ | ||
ACT1-ChIP | 5′-GATCCTTTCCTTCCCAATCTCTCTTG-3′ | ChIP |
5′-GCTCATGTAGTAGAAGATCCTATT-3′ | ||
ARN2 | 5′-GGTATGCTGCTGGAGCTGT-3′ | ChIP |
5′-AGGGCCATGAAGGTATCAA-3′ | ||
ROX1-414bp | 5′-GCAAAACAATTGGAAATCTGG-3′ | ChIP |
5′-GAACAACAAAAGAGGCAGCA-3′ | ||
ROX1-897bp | 5′-TCTACATAATGCACGAAACTTGG-3′ | ChIP |
5′-CGCAGTGTGTGTTCCTGTCT-3′ | ||
ROX1p'1000 | 5′-CTAGCATGCAGTTGACCTACATTCAAC-3′ | pROX1 |
A4-ROX1 | 5′-GGATTTCGCATCCTAGACCA-3′ | |
ROX1m Fw | 5′-TGGCGATTGAAGACAAAGAAGAAA-3′ | MUT-pROX1 |
ROX1m Rv | 5′-TTTCTTCTTTGTCTTCAATCGCCA-3′ | |
ROX1-ATG-codon | 5′-CTAGCATGCAGTTGACCTACATTCAAC-3′ | pROX1-lacZ |
5′-CTTGGATCCGGATTCATTGTTGATTGTC-3′ | ||
FET4-ORF | 5′-GAATTCCTGCAGCCCCTGTGCTTGCTGTTC-3′ | pFET4-HA |
5′-ATGTACCCATACGATGTTCCAGATTACGCTTAGCTTCATTGAACA-3′ | ||
0.5Kb-FET4-terminator | 5′-AGCGTAATCTGGAACATCGTATGGGTACATTTTTTCCAACATCAT-3′ | pFET4-HA |
5′-ACTAGTGGATCCCCCGACATATAAGCGGAG-3′ | ||
FET4m1 Fw | 5′-GCCTTCTTAATTGAGTTTAGCATC-3′ | MUT-pFET4-HA (Aft1 site 1 deletion) |
FET4m1 Rv | 5′-GATGCTAAACTCAATTAAGAAGGC-3′ | |
FET4m2 Fw | 5′-GTTCCGAAAACCCACTTTTTGTTC-3 | MUT-pFET4-HA (Aft1 site 2 deletion) |
FET4m2 Rv | 5′-GAACAAAAAGTGGGTTTTCGGAAC-3′ |