FIGURE 11.
Pre-steady-state kinetics of adenine nucleotide binding and dissociation for WT YchF and YchF(H114A). Representative time courses of the dissociation of a YchF·mant-nucleotide complex (1 μm) in the presence of excess unlabeled nucleotide (100 μm) are shown in the left panels. Representative time courses of mant-nucleotide (5 μm) association with YchF (1 μm) are shown in the right panels, with the concentration dependence of kapp on mant-nucleotide association with YchF shown in the insets. The kapp values were calculated by one-, two-, or three-exponential fitting of the time courses. A, WT YchF; B, YchF(H114A). A.U., arbitrary units.