E. coli YchF contains four histidines.
A, homology model of E. coli YchF generated with SWISS-MODEL using H. influenzae YchF (PDB ID 1JAL) as template and the amino acid sequence of E. coli YchF (UniProt accession code P0ABU2), represented as a ribbon diagram. The level of conservation of each residue is depicted using a green/white/blue color scale based on the alignment of YchF from 116 bacterial species. The four histidines present in E. coli YchF are indicated and shown as a space-filling model: His-102 (76% conserved), His-114 (98%), His-145 (17%), and His-308 (100%). B, sequence logo of the His-114-containing loop (numbering according to E. coli YchF) based on the multiple-sequence alignment in A.