TABLE 3.
Summary of experimentally determined rate constants of WT YchF and variants for binding to adenine nucleotides
| Constant | WT YchF | YchF(H114A) |
|---|---|---|
| mant-ADP | ||
| kon (μm−1 s−1) | 3.3 ± 0.5 | 8.0 ± 0.4 |
| koff (s−1) | 30 ± 2 | 58 ± 9 |
| koff (kon plot)(s−1) | 26 ± 2 | 45 ± 4 |
| KD (μm) | 9 ± 1 | 7 ± 1 |
| KD (konplot) (μm) | 8 ± 1 | 6 ± 1 |
| KD (Amp plot) (μm) | 3 ± 1 | 1.0 ± 0.4 |
| mant-ATP | ||
| kon1 (μm−1 s−1) | 0.59 ± 0.04 | 0.9 ± 0.2 |
| kon2 (s−1) | 0.15 ± 0.01 | 0.05 ± 0.02 |
| kon3 (s−1) | 1.0 ± 0.2 | |
| koff1 (s−1) | 5 ± 2 | 14 ± 3 |
| koff1 (kon plot) (s−1) | 3.4 ± 0.5 | 9 ± 2 |
| koff2 (s−1) | 0.33 ± 0.05 | 0.02a |
| koff3 (s−1) | 0.24a | |
| KD (Amp plot) (μm) | 4 ± 1 | 4 ± 2 |
a Calculated from KD.