TABLE 4.

ATPase activities of WT YchF and variants

YchF	Intrinsic kATPase	+70S (6 μm) kATPase 70S
	min−1	min−1
WT YchF	0.20 ± 0.02a	1.32 ± 0.13a
WT YchF	0.10 ± 0.01	1.24 ± 0.04
YchF(H114A)	<0.001	0.18 ± 0.03
YchF(H114R)	<0.001	0.16 ± 0.02
YchF(H114F)	<0.001
YchF(H114K)	<0.001
YchF(H102Q)	0.06 ± 0.01	1.24 ± 0.03
YchF(L76Q)	0.05 ± 0.01

^a At 37 °C