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. 2015 May 27;290(30):18650–18661. doi: 10.1074/jbc.M114.598227

TABLE 5.

Michaelis-Menten kinetic parameters of E. coli YchF(H114) variants

YchF Km kcat
μm min1
WT YchFa 7.7 ± 1.1 3.1 ± 0.2
YchF(H114A) 9 ± 7 0.7 ± 0.2
YchF(H114R) 13 ± 12 0.3 ± 0.2

a From Ref. 11.