TABLE 1.

15 purified soluble HAD-like hydrolases from S. cerevisiae and their phosphatase activity against pNPP

Gene name	ORF name	Database annotation	Reported or predicted substrate	pNPP hydrolysisa
				units/mg
Previously characterized HADs
DOG1	Yhr044c	2-Deoxyglucose 6-phosphate phosphatase 1	2-Deoxyglucose 6-phosphateb	0.9
DOG2	Yhr043c	2-Deoxyglucose 6-phosphate phosphatase 2	2-Deoxyglucose 6-phosphateb	0.6
HOR2	Yer062c	Glycerol-1-phosphate phosphohydrolase 2	Glycerol 1-phosphatec	1.1
RHR2	Yil053w	Glycerol-1-phosphate phosphohydrolase 1	Glycerol 1-phosphatec	1.3
SDT1	Ygl224c	Pyrimidine 5′-nucleotidase	5′-UMP, NMNd	9.2
Uncharacterized HADs
PHM8	Yer037w	Phosphate metabolism protein 8	Lysophosphatidic acide	17.4
PHO13	Ydl236w	4-Nitrophenyl phosphatase	p-Nitrophenyl phosphate	203.8
SER2	Ygr208w	Phosphoserine phosphatase	Phosphoserine (predicted)	1.3
UTR4	Yel038w	Enolase-phosphatase E1	5-(Methylthio)-2,3-dioxopentyl-P (predicted)	0.7
YCR015C	Ycr015c	UPF0655 protein YCR015C	No information	0.3
YKR070W	Ykr070w	Uncharacterized protein	No information	17.1
YKL033W	Ykl033w	Uncharacterized hydrolase	No information	NDf
YMR130W	Ymr130w	Uncharacterized protein	No information	0.02
YNL010W	Ynl010w	Uncharacterized phosphatase	No information	0.8
YOR131C	Yor131c	Uncharacterized hydrolase	No information	9.4

^a Data from this work; units/mg, μmol/min mg of protein.

^b Data from Ref. 41.

^c Data from Ref. 42.

^d Data from Refs. 45 and 70.

^e Data from Ref. 71.

^f ND, below the detection limit (5 nmol/min/mg of protein).