FIGURE 3.

ZO-1 recruitment and organization is not altered by depletion of N-WASP. Control and N-WASP-depleted monolayers were fixed and stained for ZO-1 and F-actin. Scale bar, 50 μm. Merge shows co-localization of ZO-1 (red) and F-actin (green) at junctional structures aligned perpendicular to the line of cell-cell contact (arrows) and separation of ZO-1 and F-actin at junctional structures aligned parallel to cell-cell contacts (arrowheads). Scale bar for merge, 25 μm. high mag, high magnification.