FIGURE 5.

N-WASP controls the organization of F-actin and VE-cadherin at endothelial cell junctions. In views of x-y projection planes, control endothelial monolayers show co-localization of F-actin (red) and VE-cadherin (green) at cell-cell junctions (arrows). N-WASP depletion results in thickened bands of VE-cadherin at cell junctions, which do not contain F-actin (arrows). Expression of full-length N-WASP restores the width of the cell junctions, defined by anti-VE-cadherin, along with the co-localization of F-actin (arrows). Scale bar, 10 μm.