FIGURE 11.
Rab11 recruits RCP to membranes. A, HeLa cells were transfected with control siRNA (siFLuc) or siRNA-duplexes targeting Rab11 and Rab14 for 72 h. The cells were transfected with GFP-RCPWT for the final 24 h and fixed. Single 0.4-μm sections were recorded with a confocal microscope. Bar, 10 μm. B, HeLa cells were transfected with control siRNA (siFLuc) or siRNA duplexes targeting Rab11 and Rab14 for 72 h. The cells were transfected with GFP-RCPWT for the final 24 h. The cells were permeabilized, fixed, mounted, and labeled with DAPI, and single 0.4-μm sections were recorded with a confocal microscope. Bar, 10 μm. C, A431 cells were transfected for 72 h with the indicated siRNA duplexes, fixed, and labeled with an antibody that recognizes endogenous RCP. Shown are single 0.4-μm sections. Bar, 10 μm. D, lysates of HeLa cells transfected with the indicated siRNA duplexes for 72 h were separated by SDS-PAGE, transferred to nitrocellulose, and immunoblotted with the indicated antibodies.