TABLE 2.
Data collection and refinement statistics
Values in parentheses correspond to the statistics for the highest resolution.
| Data collection | Rab14/RCP |
| Beamline | 24-ID-C, APS |
| Detector | ADSC |
| Space group | C2221 |
| Unit cell lengths (Å) | 95.87, 148.23, 38.81 |
| Unit cell angles (°) | 90, 90, 90 |
| Asymmetric unit | 1 × Rab14, 1 × RCP |
| Wavelength (Å) | 0.9786 |
| Low resolution limit | 80 (2.54) |
| Resolution (Å) | 2.50 (2.50) |
| Total no. of reflections | 32,904 |
| Unique reflections | 9973 |
| Multiplicity | 8.9 (5.6) |
| Completeness (%) | 96.98 (70.1) |
| Wilson B-factor (Å2) | 54.3 |
| Rmerge (%) | 9.5 (80) |
| I/σ all data | 8.5 (1.72) |
| Refinement statistics | |
| Model (chain/residues) | |
| A | 8–174; Rab14 |
| B | 595–640; RCP |
| Ramachandran map (%) | |
| Favorable + allowed | 96.6 |
| Outliers | 3.4 |
| Rwork/Rfree (%) | 22.4/29.4 |
| 2.54–2.50 Å | 30.6/36.4 |
| Non-hydrogen atoms | |
| Protein | 1,664 |
| Gpp(NH)p | 33 |
| Mg2+ | 1 |
| Waters | 16 |
| Mean isotropic B-factor (Å2) | 61.67 |
| Root mean square deviations | |
| Bond lengths (Å) | 0.008 |
| Bond angles (°) | 1.30 |