. 2015 May 31;290(30):18817–18832. doi: 10.1074/jbc.M114.612366

TABLE 3.

Light scattering analyses of Rab effectors

The following abbreviations are used: MALS, multiangle light scattering; DLS, dynamic light scattering.

Rab-effector complex	MALS	DLS	Ratio
	kDa	kDa
Rab25(7–180), MBP-FIP2	135 ± 4 (140^a)		2:2
Rab25(7–180), FIP2		65 ± 2 (60^a )	2:2
Rab14(7–175), wtRCP (581–649)		49 ± 4 (40^b)	Intermediate
Rab14(7–175), MBP-FIP2	115 ± 10 (120^c)		1:2

^a Values in parentheses indicate the expected mass of canonical 2:2 complexes of Rab25-MBP-FIP2 (multiangle light scattering) and Rab25-FIP2 (dynamic light scattering).

^b For the dynamic light scattering value for Rab14(7–175), wtRCP(581–649) is best described as “intermediate” between 1:2 and 2:2 complexes. Errors in dynamic light scattering measurements were estimated from 10 data acquisitions. The mean molar mass and associate error for multiangle light scattering were calculated as described under “Experimental Procedures.”

^c The multiangle light scattering value in parentheses for Rab14-MBP-FIP2 shows the expected mass of noncanonical 1:2 complexes. Ratio represents the stoichiometry, derived from experimental data that most closely fits the expected value (2:2 versus 1:2).