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. 2015 Jul 24;6:155. doi: 10.3389/fphar.2015.00155

FIGURE 2.

FIGURE 2

Effects of exploratory compounds on hNav1.7 channels at 0.1 Hz and 10 Hz, to determine tonic and phasic block, respectively. (A) Time course of peak sodium current amplitude in a representative HEK293 cell permanently transfected with hNav1.7, before and during application of 30 μM (S)-22b, and after drug washout. The membrane was held at –120 mV and depolarized to –30 mV for 25 ms at 0.1 or 10 Hz stimulation frequency. The numbered arrows correspond to effects described in the text. (B) Typical sodium current traces recorded in the same cell as in (A), which were obtained from the average of three records obtained at steady state in control at 0.1 Hz (corresponding to arrow 1 in A), and in presence of 30 μM (S)-22b at 0.1 Hz (arrow 4 in A) and 10 Hz (arrow 5 in A). (C–F) Representative sodium current traces recorded as in (A,B), after application of 1 mM sumatriptan, 30 μM of 20b and (R)-31b compounds, and 100 μM mexiletine.