Table 2.

Statistical analysis (ANOVA) of hNav1.7 sodium channel inhibition by exploratory compounds at the holding potential (HP) of –120 mV, at 0.1 Hz (A) and 10 Hz (B) stimulation frequencies.

	Sumatriptan		Mexiletine		20b		(R)-31b
A: 0.1 Hz
Mexiletine		yes
	yes
20b		yes		yes
	yes		yes
(R)-31b		yes		yes		ns
	yes		yes		ns
(S)-22b		yes	yes	yes	yes	ns	yes	ns
	yes		yes		yes		yes
B: 10 Hz
Mexiletine		yes
	yes
20b		yes	yes	yes
	yes		yes
(R)-31b		yes	yes	yes		ns
	yes		yes		yes
(S)-22b		yes	yes	yes	yes	yes	yes	yes
	yes		yes		yes		ns

The percentages of inhibition of sodium channels by the various drugs at each concentration, as reported in concentration–response relationships (Figure 3), were compared using ANOVA followed by ad hoc Bonferroni’s t-test. The significance is reported as “yes” (at least p < 0.05) or “ns” (not significant) in cyan cells (for comparison at 30 μM), yellow cells (100 μM), orange cells (300 μM), and pink cells (1000 μM). Note that sumatriptan was not tested at 30 μM (gray cells).