Table 1.
List of strains and plasmids used in this study.
| Strain/plasmid | Description | Source |
|---|---|---|
| Streptococcus pneumoniae | ||
| D39 | Serotype 2 strain, cps 2 | Laboratory of P. Hermans |
| RW100 | D39 ΔpsaR | Kloosterman et al. (2008) |
| MP100 | D39 ΔsczA | Kloosterman et al. (2007) |
| MP102 | D39 ΔczcD | Kloosterman et al. (2007) |
| RW104 | D39nisRKΔbgaA::PprtA-lacZ; ErmR | Kloosterman et al. (2008) |
| RW109 | D39nisRK ΔpsaR ΔbgaA::PprtA-lacZ; ErmR | Kloosterman et al. (2008) |
| IM401 | D39 ΔbgaA::PnrD-lacZ; TetR | This study |
| IM402 | D39 ΔbgaA::PpsaB-lacZ; TetR | This study |
| IM403 | D39 ΔbgaA::PpcpA-lacZ; TetR | This study |
| IM404 | D39 ΔbgaA::PczcD-lacZ; TetR | This study |
| IM405 | D39 ΔbgaA::Pcbi-lacZ; TetR | This study |
| IM451 | RW100 ΔbgaA::PpsaB-lacZ; TetR | This study |
| IM452 | RW100 ΔbgaA::PpcpA-lacZ; TetR | This study |
| IM453 | MP100 ΔbgaA::PczcD-lacZ; TetR | This study |
| Escherichia coli | ||
| EC1000 | KmR; MC1000 derivative carrying a single copy of the pWV1 repA gene in glgB | Laboratory collection |
| Lactococcus lactis | ||
| NZ9000 | MG1363 ΔpepN::nisRK | Kuipers et al. (1998) |
| Plasmids | ||
| pPP2 | AmpR TetR; promoterless lacZ. For replacement of bgaA with promoter lacZ -fusion. Derivative of pTP1 | Halfmann et al. (2007) |
| pIM301 | pPP2 PnrD-lacZ | This study |
| pIM302 | pPP2 PpsaB-lacZ | This study |
| pIM303 | pPP2 PpcpA-lacZ | This study |
| pIM304 | pPP2 PczcD-lacZ | This study |
| pIM305 | pPP2 Pcbi-lacZ | This study |
| pRW25 | pNG8048E carrying psaR-strep downstream of PnisA | Kloosterman et al. (2008) |