Molecular phylogenetic analysis of arthropod opsins by maximum likelihood method. The tree is based on MAFFT aligned full-length sequences with honeybee pteropsin as an outgroup. Bootstrap values are indicated for all nodes supported at more than 50%. The tree is drawn to scale, with branch lengths measured in numbers of substitutions per site. Opsins are colored based on optimum wavelengths: purple for ultraviolet, blue, and green for their respective clades. Accession numbers for genes used: Vanessa cardui VanG, AY613986; VanB, AY613987; VanUV, AF414074; Drosophila melanogaster Rh1, K02315; Rh2, M12896; Rh3, M17718; Rh4, M17730; Rh5, U67905; Rh6, Z86118; Rh7, NM_079311; Apis mellifera AmLop1, U26026; AmLop2, BK005515; AmBLop, AF004168; AmUVop, AF004169; Pteropsin, BK005510; Tribolium castaneum Green, M_001162519; UV, XM_965251; Megoura viciae LW, AF189714; UV_A, AF1897145; Schistocerca gregaria Lo1, X80071; Lo2, X80072; Gryllus bimaculatus Green A, HM363620; Green B, HM363621; Blue, HM363622; UV, HM363623; Dianemobius nigrofasciatus LWop, FJ232921; BLop, AB291232; UVop, AB458852; Sphodromantis spec. Opsin, X71665; Triops granaries RhA, AB293428; RhB, AB293429; RhC, AB293430; RhD, AB293431; RhE, AB293432; Hasarius adansoni Rh1, AB251846; Rh2, AB251847; Rh3, AB251848; Limulus polyphemus Opsin1, L03781; Opsin2, L03782; Opsin5, FJ791252.