Table 2.
Gene and direction | Sequence | Amplicon (bp) | Efficiency (%) |
---|---|---|---|
pGO1 forward | TATTGTACAGGCCGTCGCAG | 159 | 87.50 |
pGO1 reverse | CATGCGAAGAACCACAGAGAG | ||
pUVO forward | GGTGTGAGCATGATTCCTGC | 152 | 94.53 |
pUVO reverse | CTGGTCTCACTCTGTGTTGGTG | ||
pGO2 forward | CATTCCTCGTGTGTGGAGAA | 97 | 96.26 |
pGO2 reverse | CTAAGCCTTTTCAGCAGCTG | ||
pTRP forward | CGCATAAGAAGGCTATGGACAG | 124 | 90.07 |
pTRP reverse | CGAATCTCCATCACGTCGTC | ||
pTRPL forward | GCAGTGCTCAATTCCTCTTCAC | 164 | 93.19 |
pTRPL reverse | GGAGTCGGACTGCGGTTAAC | ||
Actin forward | GTACGTTGCTATCCAGGCTGTG | 158 | 85.60 |
Actin reverse | AATCGCGACCAGCCAGATC | ||
GAPDH forward | GTGTTCCTGTTCCCAATGTTTC | 134 | 89.51 |
GAPDH reverse | TTCAGTGTAGTCCAAGATGCC |