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. 2015 Jul 24;6:207. doi: 10.3389/fphys.2015.00207

Table 2.

Primers used for qPCR analysis.

Gene and direction Sequence Amplicon (bp) Efficiency (%)
pGO1 forward TATTGTACAGGCCGTCGCAG 159 87.50
pGO1 reverse CATGCGAAGAACCACAGAGAG
pUVO forward GGTGTGAGCATGATTCCTGC 152 94.53
pUVO reverse CTGGTCTCACTCTGTGTTGGTG
pGO2 forward CATTCCTCGTGTGTGGAGAA 97 96.26
pGO2 reverse CTAAGCCTTTTCAGCAGCTG
pTRP forward CGCATAAGAAGGCTATGGACAG 124 90.07
pTRP reverse CGAATCTCCATCACGTCGTC
pTRPL forward GCAGTGCTCAATTCCTCTTCAC 164 93.19
pTRPL reverse GGAGTCGGACTGCGGTTAAC
Actin forward GTACGTTGCTATCCAGGCTGTG 158 85.60
Actin reverse AATCGCGACCAGCCAGATC
GAPDH forward GTGTTCCTGTTCCCAATGTTTC 134 89.51
GAPDH reverse TTCAGTGTAGTCCAAGATGCC