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. 2015 Jul 24;5:12495. doi: 10.1038/srep12495

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a–c) PC-3-MDH1 or PC-3-MDH1-182 cells were injected subcutaneously into nude mice (n = 6). The overexpression of miR-182 in xenografts was confirmed by RT-PCR analysis (c). Tumors were photographed (a) and weighed (b) as indicated. (d) Western blot analysis of HIF1α protein levels in PC-3-MDH1 and PC-3-MDH1-182 xenografts. (e) PHD2 and FIH1 mRNA levels in xenografts were analyzed by RT-PCR. Data are mean ± SEM. **p < 0.01. Full-length blots are presented in Supplementary Fig. S6.