Figure 2. Fluoxetine modulates the proteomic profile in the adult mouse visual cortex.

Representative gels showing the 2D profile of the visual cortex of fluoxetine-treated (FLX) and age-matched (CTL) adult mice, stained with colloidal Coomassie Brilliant Blue G250. First dimension was performed loading 60 μg of whole protein extracts from visual cortical samples on immobilized pH 3–10 nonlinear gradient strips. Second dimension was performed on 12% SDS-PAGE gels. The 31 spots accepted as significantly differentially expressed between the two experimental conditions are indicated by a line and the corresponding match IDs (see Table 1 and Table 2). These spots were manually excised and analyzed by mass spectrometry. MW, molecular weight (kDa). To improve the clarity of the presentation gel images were cropped. Full-length uncropped images of the same gels are presented in Supplementary Figure F1.