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. 2015 Jan 29;93(6):882–892. doi: 10.1002/jnr.23556

Figure 1.

Figure 1

NO induces neuronal cell death (A) and decreases KIF5A/KIF21B gene (B) and protein (C) expression in rat cortical neurons in a time-dependent manner. 18s rRNA was used as a housekeeping gene for PCR, and KIF protein levels were normalized to GAPDH and NSE. Results are mean ± SE (n = 5 for RT-PCR experiments and n = 3 for Western blotting). *P < 0.05, **P < 0.01, ***P < 0.001. D: Immunocytochemistry for KIF proteins (red) and the nuclear marker DAPI (blue) was performed on neurons exposed to MIN or MIN with the addition of NO (MIN + N O) after 24 hr. Scale bar = 100 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]