Figure 2.

NO causes reductions in axon length and neuronal viability. Rat cortical neurons were exposed to MIN and MIN with the addition of NO for 24 hr. A: Cultures were stained for the nuclear marker DAPI (blue) and the panaxonal NF marker SMI312 (green) or NF 70 kD (green). Axonal length was measured by SMI312 (B) and NF 70 kD (C) labeling per culture field, and viable neurons (D) were counted by using DAPI positivity per field. Results are mean ± SE (n = 4). **P < 0.01, ***P < 0.001. Scale bar = 100 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]