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. 2015 Jan 29;93(6):882–892. doi: 10.1002/jnr.23556

Figure 3.

Figure 3

NO causes a time-dependent loss of NF phosphorylation and KIF expression. A: Representative immunodot blots for NF200, SMI312, KIF5A, KIF21B, GAPDH, and NSE in cortical neurons exposed to NO for different lengths of time. NO causes a time-dependent decrease in SMI312 and NF200 expression (B). Axonal phosphorylation (C) calculated by the ratio phosphorylated NF/total NF (SMI312/NF200) and KIF expression (D). E: KIF5A/NF200 and KIF21B/NF200 protein ratios in rat cortical neurons post-NO exposure. Both GAPDH and NSE were used as protein loading controls. Results are mean ± SE (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.