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. 2015 Jul 24;9:95. doi: 10.3389/fnana.2015.00095

Figure 3.

Figure 3

Detailed view of coronal paraffin sections of the oculomotor (nIII) and trochlear nucleus (nIV) in the midbrain stained for glutamate decarboxylase (GAD) in black and choline acetyltransferase (ChAT) in brown (A–F). Numerous GAD-positive puncta outline most of the motoneuronal somata (asterisks) of the superior oblique (SO) (A) the levator palpebrae muscle (LP) (B) and the subgroup containing superior rectus (SR) and IO muscles. (D) In the MR subgroups fewer GAD-positive puncta are attached to the somata (C, asterisk), but are found in the neuropile contacting cut dendrites. A considerable number of GAD-positive puncta is found around MIF motoneurons in the C-group and S-group (E,F, asterisks indicate motoneurons; arrows). Detailed views of confocal images in (G,H) show tracer labeled MR MIF motoneurons (green) in the C-group that are in close association with GAD-positive (red) puncta suggestive for direct synaptic inputs (arrowheads). MIF, multiply innervated muscle fibers. Scale bar = 25 μm in (F) (applies to A–F); Scale bar = 25 μm in (G); Scale bar = 25 μm in (H).