Table 1.
An overview of injection, fixation and immunohistochemistry details for each case.
| Case | Injection | Fixation | Sections | Immunhistochemistry |
|---|---|---|---|---|
| 1 | 3 μl CTB, MR | 4% paraformaldehyde | Frozen | CTB, CTB + GAD, CTB + vGlut1 |
| 2 | 5 μl CTB, MR | 4% paraformaldehyde | Frozen | CTB, CTB + GAD |
| 3 | 4% paraformaldehyde | Paraffin | GAD, vGlut1 or vGlut2 + ChAT | |
| 4 | 4% paraformaldehyde | Frozen | GABA-A +ChAT, GlyT2 | |
| 5 | 4% paraformaldehyde | Frozen | GlyR | |
| 6 | 4% paraformaldehyde | Frozen | GlyT2 | |
| 7 | 1% paraformaldehyde 2, 5% glutaraldehyde | Frozen | GABA | |
| 8 | 4% paraformaldehyde 0, 3% glutaraldehyde | Frozen | GABA | |
| 9 | 4% paraformaldehyde | Frozen | vGlut2 | |
| 10 | WGA-HRP | 4% paraformaldehyde | Frozen |
CTB, cholera toxin subunit B; GAD, glutamate decarboxylase; vGlut, vesicular glutamate transporter; ChAT, choline acetyltransferase; GABA, gamma-aminobutyric acid, GABA-A, GABA receptor; GlyT, glycine transporter; GlyR, glycine receptor; MR, medial rectus muscle; WGA-HRP, wheat germ agglutinin conjugated to horseradish peroxidase.