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Fluorescence rescue of deletion mutants using folding-enhancing mutations. a Rescue of whole-cell fluorescence of single deletion mutants at 37 °C using folding mutations F64L and S30R. The fluorescence of deletion mutants was normalized to that of wtGFPUV carrying corresponding folding mutations. b Rescue of whole-cell fluorescence of double deletion mutants at 20 °C by F64L mutation. Three experimental replicates were performed for each sample
