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Lysophosphatidic acid (LPA) induces Ras–protein kinase C (PKC)α interaction. SK-OV3 cells were treated with 1 µm FTI-277 or 5 µm Gö6976 or left untreated for 2 h and LPA then added to cells for 10 min followed by immunoprecipitation with PKCα mAb. Immunoprecipitates were analysed by immunoblotting to detect Ras by anti-Ras polyclonal antibody. The membrane was stripped and reprobed with-PKCα polyclonal antibody.
