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Intercepting PKCα-CARMA3 axis inhibits SK-OV3 in vitro invasion. SK-OV3 cells were infected with control Ad or Ad containing dominant-negative PKCα, CARMA3ΔCARD or Iκm, and then added into matrigel invasion chambers to analyse in vitro invasion as described in Supplementary Material. Lysophosphatidic acid (LPA, 10 µm) (a) or TNFα (50 ng ml−1) (b) was added in the underwells to stimulate cell invasion.
