Figure 10.

Expression levels of 32WT and E102G in transfected Neuro2a cells were significantly higher than that of V181A expressed in stably transfected Neuro2A cells, as determined by Western blot. Neuro2A cells were stably transfected with 32WT or the V181A or E102G mutants in the pIRES-EGFP vector as described in Materials and Methods. Protein extracts from 5 × 10⁴ cells/well were loaded in each lane, and blots were probed with Cx32 antibody as described in Materials and Methods. a, Comparison of two cell lines expressing Cx32WT (1 and 2) or the V181A mutation (3 and 4). As shown, levels of EGFP expression in the four lines are comparable. However, the level of expression of V181A is much lower than that of 32WT. b, Comparison of two lines expressing Cx32WT (5 and 6) or the E102G mutation (7 and 8). As shown, levels of expression of EGFP in the two lines are comparable as are levels of 32WT and E102G.