Expression of 32WT and the V181A and E102G mutants in Xenopus oocytes. a, Graphic representation of junctional conductance of homotypically paired oocytes injected with antisense oligonucleotide to Cx38 (Anti38) or mRNA for 32WT, or the V181A or E102G mutants determined as described in Materials and Methods; n = 44, 26, 37, and 15, respectively. b, Representative Western blot of total protein extracts from sister oocytes of those injected for assessment of junctional conductance. Levels of expression of V181A were significantly lower than those of 32WT (see Results for details).