Fig. 7.

TsSP modulates monocytes via PKCδ signaling. Control or PKCδ-deficient human monocytes were treated with TsSP (40 μg/ml) after which (a) the patrolling cell phenotype was visualized by live cell imaging (10 h) and subsequently quantified by using Image J and the expression levels of (b) CCR2, (c) LFA-1 avidity and (d) LFA-1 affinity were analyzed and quantified using flow cytometry. Cell supernatants were used to determine the levels of (e) TNF-α and (f) IL-10 secretion by enzyme-linked immunosorbent assay analysis. Experiments were performed in triplicate using 3 human control donors (a-f) and 3 (a-d) or 2 (e, f) PKCδ-deficient donors and results are presented as the mean (a, b) or the mean percentage (C-F) +/− SEM. *p < 0.05, ***p < 0.001 as determined by ANOVA and Students t test