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. 2015 Jul 25;15:545. doi: 10.1186/s12885-015-1544-y

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© Timmermans-Sprang et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Cluster analysis of different canine mammary cell lines with their relative basal gene expression. RNA from 12 different canine mammary cancer cell lines was isolated and quantitative RT-PCR was done for several target and reference genes on their cDNA. RT-PCR data were analyzed in RStudio with the Pearson:Spearman test and graphically presented with their corresponding TOP/FOP ratios (blue >30; dark grey 2-6; light grey <2) as published previously [5]. The three cell lines with high basal TOP/FOP ratios (canonical Wnt pathway) cluster together in the non-hierarchical cluster analysis. Green is a high expressed gene and red is low expressed gene