Fig. 5.

Effects on gene expression. CMT1, CMT-U27 and CMT9 cells were grown for 40 h with the three inhibitors, 50nM BEZ235 (a), 100nM Everolimus (b) and 20 μM Src-l1 (c). After inhibition RNA was isolated and the relative expression of several target genes was measured by quantitative RT-PCR. The differences in expression levels compared to an appropriate control were expressed as the mean of 12 samples out three independent experiments. *P < 0.01