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. 2015 Jun 25;137(28):9053–9060. doi: 10.1021/jacs.5b03912

Figure 2.

Figure 2

pH-Dependent CD spectra and ionic strength-dependent Tm values for the human telomere i-motif. (A) The CD spectra were recorded on a 10 μM solution of the sequence 5′-CCC TAA CCC TAA CCC TAA CCC-3′ in a 25 mM three buffer system (acetate, phosphate, and borate) with 1.0 M KCl at 20 °C. The pH values were adjusted from 5.0 to 7.5 in 0.25 pH unit increments. The inset represents the molar ellipticity at 286 nm vs pH that determined the transition pH (pHT) to be 6.1. (B) Table of measured Tm values for the i-motif sequence at physiological ionic strength (140 mM KCl and 12 mM NaCl) vs the high ionic strength for the nanopore analysis (1.0 M) at pH 5.0. Values were determined by monitoring the temperature-dependent unfolding process by UV–vis at 295 nm.