Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 16;43(13):6373–6383. doi: 10.1093/nar/gkv302

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Effect of added DNA polymerases. (A) The effect of added Klenow fragment. The template was the 35-nt telomere DNA (TO35). The reaction conditions were identical to Figure 2B except for 4 h of reaction time and the addition of Klenow fragment (0.2 units; even-numbered lanes). The products were electrophoresed in 15% SDS-PAGE directly (upper panel) or after heat denaturation (lower panel). [α³²P]-dCTP (0.165 μM) was supplemented with 10 μM non-radioactive dCTP, dATP, dGTP and dTTP as indicated. (B) The effect of added DinB1. Left panel. The polymerase activity of purified DinB1 was demonstrated by the fill in reaction on the MulI–NdeI restriction fragments (‘MN’; Figure 2B). The reaction conditions were identical to those in Figure 2B. Right panel. The extension activity on the in vitro deoxynucleotidylated product by DinB1 was tested under the same conditions as in (A). ‘Kle’, Klenow fragment; ‘Din’, DinB1; ‘–’, none added.