Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 8;43(13):e88. doi: 10.1093/nar/gkv464

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Characterization of the native promoters using a YFP-mCherry dual reporter system. (A) The YFP-mCherry reporter system. Each promoter was inserted upstream of the YFP gene in a plasmid, which also contains an mCherry fluorescent protein driven by the TEF2 promoter and terminator. The activity of a given promoter was defined as the ratio between the YFP and mCherry fluorescence intensity. (B) High correlation between repeated measurements (R² = 0.96). Each dot represents a promoter, which was measured in two independent experiments. (C) The distribution of promoter activities. The strength of each promoter was normalized to that of CYC1 and categorized into six arbitrary groups. (D) Change of promoter activity under different stress conditions. The activity of each promoter was compared to that in the normal condition (in SC-Leu medium). Shown here is a total of 71 promoters, which were tested in the first batch of experiments.