Table 2. The efficiency of part assemblya.
| Parts | Plate ID | PCR amplification of parts from gDNA (%) | Confirm insertion by colony PCR (%) |
|---|---|---|---|
| PRO | ChrI_1_PRO | 90.6 | 84.3 |
| ChrIII_1_PRO | 91.7 | 91.7 | |
| ChrIII_2_PRO | 74.0 | 90.6 | |
| ChrVII_2_PRO | 97.9 | 82.3 | |
| ChrVII_3_PRO | 84.4 | 91.7 | |
| ChrVII_4_PRO | 84.4 | 78.1 | |
| ChrVII_5_PRO | 94.8 | 79.2 | |
| ChrVIII_2_PRO | 70.8 | 68.8 | |
| ChrVIII_3_PRO | 85.4 | 72.9 | |
| ChrXII_1_PRO | 88.5 | 85.4 | |
| ChrXII_3_PRO | 96.9 | 82.3 | |
| ChrXII_4_PRO | 94.8 | 87.5 | |
| ChrXII_5_PRO | 83.3 | 87.5 | |
| ChrXII_6_PRO | 99.0 | 93.8 | |
| ORF | ChrI_1_ORF | 59.4 | 77.1 |
| ChrXII_1_ORF | 77.1 | 70.8 | |
| TER | ChrI_1_TER | 87.5 | 96.9 |
| ChrXII_1_TER | 95.8 | 93.8 | |
| Average | 86.4 | 84.2 |
aThe efficiency was calculated based on a single round of assembly and listed as the percentage of positive PCR at the expected size. Only the plates with 96 pairs of primers were used in the calculation.