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| Assay method | β-Carotene synthesis genes | Violacein synthesis genes | Protein complex genes |
|---|---|---|---|
| Colony PCR | 21/24 (87.5%) | 18/20(90%) | 28/30(93.3%) |
| Digestion | 100% | 100% | 100% |
aThe efficiency listed here is for parts without internal BsmBI sites. For those containing one or more internal sites, the efficiency decreased dramatically if the same protocol was used.
