Table 4. The efficiency of pathway assemblya.
| Promoters | Terminators | # of total clones (LEU+) | # of clones (LEU+URA-HIS-) | Confirmed by PCR |
|---|---|---|---|---|
| Individual | Same | 13701 | 160 (1.17%) | 54/56 (96.43%) |
| Different | 170 | 89 (52.35%) | 6/6 (100%) | |
| Pooledb | Same | 1112 | 41(3.69%) | 3/4 (75%) |
| 2416 | 76(3.15%) | 13/13 (100%) | ||
| 32 | 8(25%) | 6/7 (85.7%) | ||
| Different | 160 | 69 (43.13%) | 20/20(100%) | |
| 177 | 49 (27.68%) | |||
| 199 | 60 (30.15%) | |||
| 231 | 62 (26.83%) | |||
| 229 | 65 (28.39%) |
aThe efficiency listed here is for pathways assembled in vitro and integrated into the yeast genome directly.
bA total of 151 promoters were pooled together.