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. 2015 Jun 3;43(13):6257–6269. doi: 10.1093/nar/gkv568

Figure 6.

Figure 6.

Both SOX9 and NF-Y are required for transcriptional regulation of target genes. (A) siRNA-mediated NF-YA and SOX9 knockdown in HCT116 cells were confirmed by western analysis. (B) SOX9 ChIP-qPCR analysis showed that both NF-YA knockdown cells and SOX9 knockdown cells resulted in decreased binding affinity of SOX9 to CCNB1 and CDK1 promoters compared to the cells treated with control siRNA (Ctrl). (C) NF-YA ChIP-qPCR analysis showed that decreased NF-YA expression resulted in reduction of NF-YA binding affinity to CCNB1 and CDK1, however, SOX9 knockdown enhanced NF-YA binding affinity to these genes. (D) In NF-YA knockdown cells, expression of the corresponding mRNAs was downregulated. Upon SOX9 knockdown, although NF-YA binding affinity was enhanced, expression of the corresponding mRNAs was downregulated. *P < 0.05, **P < 0.01, ***P < 0.001.