Figure 3.
EZH2 and H3K27me3 are found at the promoters of TFIIIC target genes. (A and C) Displayed were the products of qPCR from the ChIP assays analyzed in agrose gel. ChIP assays were performed using EZH2 and H3K7me3 antibodies with indicated primers. Normal mouse IgG was used as negative control. (B and D) ChIP assays using MCF7 or SUM159 lysates were carried out followed by detection by qPCR. Results were presented by mean ± S.D. for three independent experiments. (E and F) SUM159 cells were transfected with the specific EZH2 siRNA 2 or control siRNA. Results of ChIP assays were detected by qPCR for TFIIIC targets including tRNATyr, 5S rRNA, 7SL RNA and MYT-1. MYT-1 is a known EZH2 target and was used as a positive control. Knockdown efficiency was detected by western blot with an EZH2 antibody. Results were presented by mean ± S.D. for three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 by Student's t-test.