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. 2015 Jun 1;43(13):6439–6449. doi: 10.1093/nar/gkv579

Figure 2.

Figure 2.

Interaction of poly-(dG:dC)/(G:C) with the cytoplasmic sequence of LAMP2C. (A) DsDNA sequences used for pull-down assays. (B and C) Interaction of the cytosolic sequence of LAMP2C with poly-(dA:dT)(30) and poly-(dG:dC)(30). Pull-down assays were performed using DNA (1 nmol) (A) and biotinylated peptide. The DNA bound to the cytosolic sequence of LAMP2C was assessed by electrophoresis in agarose gels, followed by EtBr staining (B). Relative levels of bound DNA were quantified by OD260 measurement (C). Mean values are shown with SEM (n = 3). **P < 0.01, n.s., not significant (Tukey's test). (D) DsRNA sequences used for pull-down assays. (E and F) Interaction of the cytosolic sequence of LAMP2C with poly-(A:U)(15) and poly-(G:C)(15). Pull-down assays were performed using RNA (2 nmol) (D). The RNA bound to the cytosolic sequence of LAMP2C was assessed by electrophoresis in agarose gels, followed by EtBr staining (E). Relative levels of bound RNA were quantified by OD260 measurement (F). Mean values are shown with SEM (n = 3). **P < 0.01 (Tukey's test).