Figure 6.

Impact of terminal loop mutations on translational repression in vivo. A plasmid encoding tnpA^PA-6-lacZ or tnpA^PA-6/M1’-lacZ was co-transformed into DBH323 with a compatible plasmid expressing art200 (WT or M1) in trans to tnpA-lacZ or an empty vector control. β-galactosidase activity was measured in cells grown to mid-exponential phase in LB media. Bars show the mean expression from two independent experiments and error bars indicate standard error on the mean (n = 6). Thebottom panel shows primer extension analysis using RNA extracted from cells grown in the Miller assay (top panel). lpp was used as a loading control.