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. 2015 Jun 4;43(13):6511–6527. doi: 10.1093/nar/gkv584

Figure 7.

Figure 7.

Impact of tnpA lower stem (LS) mutations on transposase expression. (A) The toeprinting assay was performed on tnpA RNA (WT and LS; 200 nM) in the presence or absence of art200 (WT, LS’ or M1; 3 μM) as described in Figure 5. The toeprint signal spans nucleotides 15–17. C, U, A and G are sequencing lanes. (B) Toeprint signal band intensities (G+15, A+16 and G+16) from (A) were quantified. The toeprint signal for tnpAWT in the absence of art200 was set at 100%. (C) A plasmid encoding tnpAWT-lacZ or tnpALS-lacZ was co-transformed into DBH323 with a compatible plasmid expressing art200 titrator RNA (WT or M1) or an empty vector control. β-galactosidase activity was measured in cells grown to mid-exponential phase in LB media. Bars show the mean expression from two independent experiments and error bars indicate standard error on the mean (n = 6).