. 2015 Jun 4;43(13):6511–6527. doi: 10.1093/nar/gkv584

Table 1. Strains and plasmids.

Name	Description	Notes
E. coli
DBH13	HB101 [F- leu- pro-]; Sm^R	Recipient strain for mating out experiments
DBH33	NK5830 [recA- arg -ΔlacproXIII nalR rifR/ F’ lacpro+]	Parent strain for mating out donor
DBH291	DBH33 λDBH881 (Mini IS200-kan); Kan^R	Donor strain for mating out experiments
DBH323	MC4100 ΔrecA774::kan; Sm^RKan^R	Miller assays (hfq⁺) (36)
DBH326	DBH323 hfq-1::cat; Sm^RKan^RCm^R	Miller assays (hfq⁻) (36)
Plasmids
pDH857	pBAD24-SD_BAD24-tnpA^WT; Ap^R	TnpA expression for mating out, pBAD24 regulatory elements
pDH860	pBAD24-SD_BAD24-tnpA^Y125F; Ap^R	TnpA^Y125F (cat. dead) expression for mating out, pBAD24 regulatory elements
pDH861	pGEM-T Easy derived, tnpA-lacZ TLF; Ap^R	tnpA^WT-lacZ TLF
pDH862	pDH861 with lower-stem mutations, tnpA^LS-lacZ; Ap^R	TLF with disrupted stem-loop structure
pDH880	pDH861 with P_A-6 mutation; Ap^R	tnpA-lacZ, no cis-art200
pDH896	pBAD24-tnpA^WT; Ap^R	TnpA expression for mating out, WT IS200 5'UTR
pDH897	pBAD24-tnpA^LS; Ap^R	TnpA expression for mating out, LS IS200 5'UTR
pDH898	pDH900 with T7 P_A1-tnpA_45–298; Tet^R	High-copy titrator, no cis-art200
pDH899	pDH900 with P_Tet-tnpA_45–298; Tet^R	Low-copy titrator, no cis-art200
pDH900	pACYC184 derivative; Tet^R Cm^S	Vector control
pDH902	pDH900 with IS200₄₅–₂₉₈; Tet^R	trans-art200
pDH912	pDH902 with M1 mutations; Tet^R	trans-art200^M1
pDH914	pDH899 with M1 mutations; Tet^R	Low-copy titrator^M1’
pDH916	pDH861 with M1 mutations; Ap^R	tnpA^M1’-lacZ TLF
pDH918	pDH880 with M1 mutations; Ap^R	tnpA^M1’-lacZ TLF, no cis-art200