Figure 2.

Overview of m⁶A methylome in Escherichia coli. (A) The m⁶A peak distribution within different gene contexts. The y-axis represents (number of reads/length unit)/(number of total reads), which is an indicator of the extent to which sequencing reads are enriched in different segments across the entire transcript. (B) Accumulation of m⁶A reads along transcripts. Each transcript is divided into three parts: Overlap Start, Inside and Overlap End. (C) The UGCCAG conserved sequence motif for m⁶A-containing peak regions. (D) Pie chart displaying the percentage of genes containing m⁶A peaks with functional categories. (E) GO-enrichment analysis of all the genes with m⁶A peaks. The effect size (number of enriched genes/total genes in the GO category) for each category is 15/45 (aerobic respiration), 15/122 (amino acids biosynthesis), 6/138 (response to abotic stimulus), 5/61 (cell wall biosynthesis) and 6/39 (anaerobic respiration), respectively. The statistical test (P-value) used by DAVID was the Fisher Exact test.