Skip to main content
. 2015 Jun 18;43(13):6568–6578. doi: 10.1093/nar/gkv617

Figure 3.

Figure 3.

3′-Tailed mirtrons effect strong knockdown when targeted to the coding region. (A) Sequence of TMirtFL19 compared to MirtFL19. Mutations made to the 5′ splice site (US) from GUGAG to AUGAG and branch point (BP) from CTCAG to CTCCC are indicated by substitutions of the nucleotides within the boxes. Nucleotides in italics denote guide strand, uppercase denotes branch point. (B) HEK293 cells were transfected with 100 ng of psiCheck2.2 and 0, 250 or 800 ng of peGFP-MirtFL19 or peGFP-TMirtFL19, and topped up to 900 ng of nucleic acids with peGFP-NAD. Cells were harvested two days after transfection. (C) HEK293 cells were transfected with 100 ng of psiCheck2.2, and 500 ng of mirtrons/NAD. Cells were harvested two days after transfection. (D) HEK293 cells were transfected with 100 ng of psiCheck2.2, and 500 ng of mirtrons / NAD. Cells were harvested 2 days after transfection. *P < 0.05, **P < 0.01, ***P < 0.001 versus NAD control, n = 3 biological replicates for each experiment unless otherwise stated. Error bars reflect standard deviation.