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. 2015 Jun 18;43(13):6359–6372. doi: 10.1093/nar/gkv621

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The loss of Smarcal1 reduces the efficiency of V(D)J recombination without compromising its fidelity. (A) The structure of two episomal V(D)J-recombination substrates, pJH200 and pJH290, and their recombination products. Open triangles and closed boxes represent recombination signals (RSs) and V(D)J-coding sequences, respectively. Cam^R = chloramphenicol-resistance gene; P = promoter. (B) Schematic representation of the experimental method for the V(D)J-recombination assay. Frequency of recombination was calculated by dividing the number of rearranged products (the number of cam^R colonies) by the number of recovered plasmids (the number of ampicillin-resistant [amp^R] colonies). (C) Recombination frequency of TK6 cells carrying the indicated genotypes. Data shown are the means of more than three experiments. Error bars indicate SD of more than three independent experiments. P-value was calculated by Student's t-test. The total number of ampicillin- and chloramphenicol-resistant colonies is shown in the right panel. Supplemental Table S2 shows the nucleotide sequences of coding joints associated with deletion events.