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. 2015 Jun 18;43(13):6359–6372. doi: 10.1093/nar/gkv621

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The loss of Smarcal1 results in compromised accumulation of Ku70, DNA-PKcs and XRCC4 at DSB sites. (A) Western blot data showing the validation of fractionation of the cytoplasmic (Cyto), nuclear soluble (NS) and chromatin-bound (CB) fractions isolated from the whole-cell extract (WCE). (B) Western blot data show the accumulation of XRCC4 (upper panel) and Ku70 (lower panel) in the chromatin-bound fraction after one-hour exposure of cells to ICRF193. (C) Histogram showing the quantification of XRCC4 and Ku70 in (B). The y-axis represents the amount of the chromatin-bound fraction relative to the total amount of the chromatin-bound fraction plus the nuclear soluble fraction. (D) Downward arrows represent the I-Sce1- (upper) and the TALEN- (lower) cutting sites in the TK and p53 genes, respectively. Pairs of horizontal opposing arrows indicate the sets of primers for quantitative real-time PCR. (E) Histograms represent the accumulation of XRCC4 near the I-Sce1-induced DSB in the TK locus (upper panel) and near the TALEN-induced DSB at the p53 locus (lower panel). (F) Histogram represents the accumulation of Ku70 near the I-Sce1-induced DSB in the TK locus. (G) Histogram represents the accumulation of DNA-PKcs near the I-Sce1-induced DSB in the TK locus.