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. 2015 Jun 22;43(13):6486–6499. doi: 10.1093/nar/gkv633

Figure 3.

Figure 3.

SAMHD1 is a single-stranded nucleic acid binding protein. (A) Binding of SAMHD1 to a 40mer 5′ FAM-labeled ssRNA (10 nM) and a 40 bp RNA:DNA hybrid duplex (10 nM) were measured by following the increase in fluorescence anisotropy of the FAM fluorophore upon nucleic acid binding. (B) Electrophoretic mobility shift (EMSA) measurements of SAMHD1 binding to ssRNA40 (10 nM). (C) The binding of SAMHD1 to a 57mer 5′ FAM-labeled ssDNA (50 nM) and a 57 bp DNA duplex (50 nM) were measured by following the increase in fluorescence anisotropy of the FAM fluorophore upon nucleic acid binding. (D) EMSA measurements of SAMHD1 binding to ssDNA57 (50 nM).