Table 1. Molecular characterization of SDR5C1 mutant forms.
| Dehydrogenase activity (units/mg)a | RNase P activity, kobs (min−1)b | m1G9 methyltransferase activity, kobs (min−1)b | Tetramerized SDR5C1a,c | Complexed TRMT10Ca,d | |
|---|---|---|---|---|---|
| wild type | 33.33 ± 3.89 | 4.63 ± 0.61 | 1.05 ± 0.16 | 100% | 43.2% ± 1.7 |
| R130C | 6.90 ± 1.78 | n.d. | n.d. | 100% | 28.7% ± 3.5 |
| P210S | 8.21 ± 2.29 | 0.59 ± 0.07 | 0.12 ± 0.02 | 100% | 39.7% ± 0.6 |
| R226Q | 0.58 ± 0.09 | n.d. | n.d. | 26.0% ± 9.5 | 14.3% ± 4.0 |
| N247S | 0.67 ± 0.21 | n.d. | n.d. | 25.8% ± 6.1 | 9.3% ± 6.7 |
| K172A | 0.63 ± 0.28 | 5.13 ± 0.60 | 0.90 ± 0.18 | 100% | 43.0% ± 3.5 |
Summary of SDR5C1-dependent enzymatic activities, SDR5C1 tetramerization, and interaction with TRMT10C. Data as illustrated in Figures 1, 2, and 4 (n.d., not determined); corrected values in bold.
aMean and SD.
bRate and standard error derived by non-linear regression.
cEstimated from four independent blue native PAGE experiments.
dMass percentage of the complex.